A Steroid, 7α-hydroxy-β-sitosterol from the Bark Chisocheton macrophyllus (Meliaceae)

Abstract

The compound 7α-hydroxy-β-sitosterol has been isolated from the bark of Chisocheton macrophyllus. This compound was obtained by total maceration with methanol and continued partitioning with the solvents n-hexane, ethyl acetate, and n-butanol. The n-hexane extract was separated and purified using various chromatographic techniques to obtain compound 1. Determination of the structure of compound 1 was identified using various spectroscopic techniques such as MS, IR, 1D NMR, and 2D NMR, as well as comparison of NMR data with reference compounds. Compound 1 was tested for its cytotoxic activity against MCF-7 breast cancer cells and was found to have a weak IC₅₀ value of 276.57 µg/mL.